On the resulting heating curve, there were three peaks that characterise endothermic transitions. One around 30°C, one at 87°C and one at 207°C. As a phase transformation slightly above room temperature seems unlikely given the polymer properties,  the first peak was considered as an artefact linked to the onset of the DSC, and thus erased from the DSC curve .

The second peak is quite interesting: at this temperature, one would expect to see a glass transition, but it is not apparent at first sight. However, when one extends the baselines at both edges of the peak, a shift appears: this is the glass transition. The endothermic peak that follows the glass transition characterises an enthalpic relaxation [7]. The material was dried overnight at 60°C (which is close but still below Tg) and then left to (slowly) cool in desiccator for several hours: this can be assimilated to physical aging the material. Upon aging, the enthalpy progressively decreases. Indeed, when an amorphous material is in its glassy state, it is in non-equilibrium froze liquid like state with an excess of thermodynamic properties like enthalpy and volume. When held close but below Tg for a long time, this non-equilibrium glass tries to attain a lower energy equilibrium. Physically, the polymer chains, which still retain some mobility, will rearrange, rendering the material more brittle [9]. The dissipated enthalpy is recovered upon heating the material above Tg and takes the form of an endotherm at the glass transition [7]. The presence of an enthalpic relaxation make the precise identification of Tg difficult. However, if one draws a line between both edges of the endotherm and measures its middle, one obtains a midpoint glass transition temperature of ~85°C. This is thus as expected.

The third and last peak is smaller, but cannot be ignored (even though this peak is unexpected). This endotherm can be associated to the melting of the filament, making the material semicrystalline instead of amorphous. The melting peak is quite broad. The area below it is related to the amount of crystalline material in the polymer [8]. However, the percentage of crystallinity could not be determined as a reference value is needed. Indeed, the crystallinity of a material is obtained by normalizing the observed heat of fusion to that of a 100 % crystalline sample of the same polymer. However, such a polymers are often rare, and therefore literature value are used. In the case of nGen, as the exact composition of the material is unknown, literature values were not available and thus the crystallinity of the material could not be determined [8].